Unravelling the genetic regulation of enzyme secretion in hypercellulolytic strains of the filamentous fungus Trichdoerma reesei

Status
closed
Location

Industrial large-scale production of lignocellulolytic enzymes by Trichoderma reesei represents a major economic issue for ethanol production from lignocellulosic biomass. In particular, the capacity of this ascomycete to secrete large amounts of enzymes is primordial. Hyperproducing strains are able to develop an extended endoplasmatic reticulum if they are cultured in the presence of substrates which induce enzyme production. However, despite sequencing of wild type and hyperproducing strains, a complete explanation for this phenotype based on the genetic modifications caused by random mutations is still missing. In the present PhD project, we want to identify the responsible genes for this highly developed secretion system. As a first step, transcriptomic data of T. reesei and other fungal species (from our own laboratory and published data) will be analysed in order to identify new players of the secretion system including regulatory pathways. The results will then be used to construct mutant strains with a modified secretion capacity which will be studied on a phenotypic and a transcriptomic level. A second part of this thesis will therefore be dedicated to random mutagenesis of strains with a low or impaired secretion capacity and screening for increased secretion on non-inducing carbon sources. The results should contribute to a better understanding of the mutations underlying the hypersecretion phenotype of industrial strains and unblock new approaches for improvement of secretion of lignocellulases and other proteins in T. reesei. 

Keywords: Trichoderma reesei, lignocellulolytic enzymes, secretion, transcriptome, bioethanol

  • Academic supervisor    Dr MARGEOT Antoine, Dép. de Biotechnologie, IFPEN
  • Doctoral School    ED581 ABIES/ AGRICULTURE, ALIMENTATION, BIOLOGIE, ENVIRONNEMENT, SANTÉ (www.agroparistech.fr)
  • IFPEN supervisor    Dr. Senta Blanquet, Dép. de Biotechnologie, senta.blanquet@ifpen.fr
  • PhD location    IFP Energies nouvelles, Rueil-Malmaison (near Paris), France
  • Duration and start date    3 years, starting not earlier than November 2020
  • Employer    ADEME, Paris, France
  • Academic requirements    Master’s degree in biology. The candidate should have very good theoretical and practical knowledge of molecular biology and genetics.
  • Language requirements    Fluency in English, basic knowledge of French
  • Other requirements    Knowledge of bioinformatics (especially in treatment of –omics data) is a plus
Contact
Encadrant IFPEN 
BLANQUET Senta
Dép. de Biotechnologie
Texte libre

IFP Energies nouvelles is a French public-sector research, innovation and training center. Its mission is to develop efficient, economical, clean and sustainable technologies in the fields of energy, transport and the environment. For more information, see https://www.ifpen.com. 
IFPEN offers a stimulating research environment, with access to first in class laboratory infrastructures and computing facilities. All PhD students have access to dedicated seminars and training sessions.